How to Measure Caffeine in Coffee

crazy by Editorial Staff | Posted on December 16th, 2022

Coffee is one of the world’s favorite beverages, but did you know that you can measure the amount of caffeine in it? Whether you’re a coffee lover or just curious about how much caffeine is in your cup, this blog post will teach you all about measuring caffeine.

We’ll cover which tools and techniques to use, plus some tips for ensuring your measurements are accurate. So grab a cup of joe, and let’s get started!

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An average cup of joe may have around 95 mg of caffeine. However, this amount varies between drinks and ranges from almost zero to 500 mg.

The figures below will give you a rough idea of the caffeine in coffee.

  • Brewed Coffee (8 oz / 237 mL): 95-200 mg of caffeine
  • Latte (8 oz / 237 mL): 63-175 mg of caffeine
  • Decaffeinated Brewed (8 oz / 237 mL): 2-12 mg of caffeine
  • Espresso (1 oz / 30 mL): 47-75 mg of caffeine
  • Decaffeinated Espresso (1 oz / 30 mL): 0-15 mg of caffeine
  • Instant (8 oz / 237 mL): 27-173 mg of caffeine
  • Decaffeinated Instant (8 oz / 237 mL): 27-173 mg of caffeine

Check out our article If you want to know more about caffeine counts for coffee drinks and beans from different regions.

Why does the amount of caffeine vary?

Coffee is one of the most popular beverages in the world, and its caffeine content can vary significantly. Depending on the type of coffee, roast, brewing method, and grind size, the amount of caffeine in a cup of coffee can range from as little as 30mg to as much as 500mg. But why does the amount of caffeine vary?

Is caffeine something to worry about?

Caffeine is no cause for concern unless you exceed the recommended daily limit. Typically, consuming 400–600 mg/day of caffeine is healthy. However, it is good to note our bodies react differently. So it is good to experiment and see which amount is ideal for you. 

What factors affect caffeine content?

The type of coffee bean used is one of the main factors in determining the amount of caffeine in a cup of coffee.

The amount of caffeine in your cup depends on many factors. They include:

  • Type of coffee: The caffeine content can vary significantly between regularly brewed coffee, espresso, instant coffee, and decaf coffee.
  • Type of coffee beans: Many coffee beans are available, which may naturally contain different amounts of caffeine.
  • Roasting: Lighter roasts have more caffeine than darker roasts, although the darker roasts have a deeper flavor.
  • Serving size: “One cup of coffee” can range anywhere from 30–700 ml (1–24 oz), significantly affecting the total caffeine content.

How does the roast level affect caffeine?

Some people insist dark roasted coffee has more caffeine. Others maintain that light roasts have more caffeine. Who is right? Here is what you need to know.

During the roasting process, coffee’s caffeine content remains relatively stable. So, what changes are the taste and caffeine levels per the beans’ weight and volume? This is because roasted beans lose some of their moisture and overall mass.

Beans roasted dark or past the second crack will have a conspicuously lighter weight than light roasts. That decrease in density and mass is another reason why some coffee lovers believe dark roasted coffees lack caffeine.

Different methods of measuring the total caffeine content

Measuring the total caffeine content in coffee beans is an important part of understanding the quality and flavor of the final cup of coffee. It is necessary to determine how much caffeine is extracted from the grounds during brewing to ensure that the amount of caffeine in a cup of coffee is within acceptable levels.

There are a few different methods of measuring the total caffeine content in coffee beans, including UV spectroscopy, HPLC, and peak area measurements.

The Volumetric Method for determining total caffeine content

The volumetric method is a fast and cost-effective way of accurately measuring the total caffeine content in coffee. It involves the use of a spectrophotometer, which is an instrument that measures light absorption, to measure the concentration of caffeine in a sample of coffee. This method is simple and can be carried out in a laboratory setting.

The first step in the process is to prepare a standard solution for the spectrophotometer. This solution must contain a known concentration of caffeine and is used to calibrate the instrument. The standard solution should then be placed in the spectrophotometer to measure light absorption at a specific wavelength.

The next step is to prepare a sample solution with coffee extract. The sample solution should be accurately measured and placed in the spectrophotometer. The instrument will then measure light absorption at the same wavelength as that used to calibrate the instrument with the standard solution.

The caffeine content of the sample solution can then be determined by comparing the measurements from both solutions. The volumetric method requires accurate measurements of both solutions and careful spectrophotometer calibration to ensure reliable results.

Overall, the volumetric method is a quick, easy, and cost-effective way of accurately determining the total caffeine content in coffee. It can be used for research and quality control purposes in commercial settings.

Assessing extraction efficiency with an Ultraviolet Spectrophotometer

The extraction efficiency of caffeine from coffee beans can be accurately measured using an Ultraviolet (UV) spectrophotometer. This instrument uses UV light to detect the amount of caffeine present in a sample. It works by measuring the UV light absorption of a sample compared to a reference sample.

The results obtained from this method are highly reliable and accurate. The extraction efficiency of caffeine from coffee beans can be determined by measuring the absorbance of the sample at two or more different wavelengths. This helps to identify the amount of caffeine in the sample and compare it with the reference sample.

This method is particularly useful for determining the quality and quantity of caffeine in coffee beans. It helps to ensure that the right amount of caffeine is extracted from the beans, helping maintain the desired flavor and aroma of coffee.

Titration as a method for determining concentration levels of caffeine

Titration is a method used to measure a substance’s concentration in a solution accurately. One of the most common titration uses is determining the caffeine concentration in coffee. By using an automated titration device, it is possible to determine the caffeine content of coffee precisely.

The process begins by preparing the sample and dissolving it in water. Then, an excess amount of iodine is added to the sample, and the mixture is stirred. The iodine reacts with the caffeine molecules in the coffee, forming a colored compound.

As more iodine is added, the color intensity increases and eventually reaches a point where it can be measured using a spectrophotometer. The amount of caffeine in the sample can then be calculated using a predetermined formula.

This method is advantageous because it is relatively fast and environmentally friendly and involves minimal use of organic solvents. Additionally, titration is a very accurate method for measuring caffeine content, as it allows for precise control over the reaction’s endpoint. Furthermore, titration eliminates any potential errors caused by gradual changes in color during the reaction.

Overall, titration is an ideal way to measure caffeine levels in coffee, as it is fast, accurate, and eco-friendly. It also eliminates any potential misjudgments that may occur during manual measurements. With its ability to accurately measure caffeine content, a titration is an invaluable tool for those looking to measure caffeine content in coffee.

HPLC and Mass Spectrometry Methods for detecting low levels of caffeine

High-performance liquid chromatography (HPLC) and mass spectrometry (MS) are two of the most powerful and reliable methods for measuring low levels of caffeine in coffee. HPLC is a type of chromatography that separates and quantifies the components in a sample based on their chemical and physical properties.

The technique uses a mobile phase, typically consisting of either an organic solvent or a mixture of solvents, flowing through the column to separate the analytes. Once the analytes have been separated, they can be detected using UV or fluorescence detectors.

On the other hand, mass spectrometry is an analytical technique that measures the mass-to-charge ratio of molecules. It is commonly used to identify unknown compounds in a sample and quantify known compounds’ concentrations. It works by ionizing the analytes and then measuring how they are split up by a powerful electric or magnetic field.

These two methods provide precise and accurate readings for determining the caffeine content of coffee. They can also detect very low levels of caffeine, making them ideal for determining caffeine content in coffee for regulatory purposes.

In addition, HPLC and MS are relatively non-destructive, meaning that samples can be re-analyzed multiple times without damaging them. This makes them especially well-suited for quality control and other research applications.

Testing instant coffees and decafs with Laser Diffraction Particle Size Analysis

Testing instant coffees and decafs for caffeine levels can be done through Laser Diffraction Particle Size Analysis. This analysis involves using a laser beam to measure the size of particles suspended in a sample. The size of the particles can then be used to determine the amount of caffeine in the sample.

The laser beam is directed through the sample, and a detector measures the scattered light from particles. The scattered light is then used to create a particle size distribution, which can be used to determine the amount of caffeine present in the sample. This method is highly accurate and allows for rapid analysis, making it ideal for testing instant coffees and decafs for caffeine levels.

Can you measure caffeine content yourself?

If you want to test your coffee beans for the amount of caffeine it has, I am sorry to disappoint. Many companies can measure the caffeine content in their drinks. They use ultraviolet spectroscopy — which is very accurate but expensive. 

There is also a chemical way to measure the caffeine content in your coffee. Unfortunately, it is a lengthy process that involves using different materials, some of which are toxic, like sodium hydroxide. That means there isn’t any coffee left to enjoy when done. Learn more about how Maximillian Du chemically measured caffeine in coffee and other drinks. 

How do you minimize or maximize your caffeine intake?

Your beans’ measurement and grind size dictate the amount of caffeine in your cup. For example, since dark roast coffees weigh less than light roast coffees, a pound of dark roast will include more beans than a pound of light. 

If you measure by weight, the dark roast coffees will have more caffeine. However, a heavier and denser light roast will produce more caffeine if you measure your beans by volume. For example, if you measured out 20 grams of dark and light-roasted beans, both will weigh the same, but the cup of the dark roast will have more caffeine because it requires extra beans. Sound confusing? You are not alone. I also had to reread it a few more times!

Once your coffee is measured, you can focus on the water temperature, brew time, and grind levels. To explain this in the simplest form possible, the finer the grind, the longer the brew time, and the hotter the temperature, the more caffeinated your brew will be. 


You shouldn’t be worried about your caffeine consumption if you are not going overboard. However, you must be cautious if you have a condition requiring you to watch your caffeine intake. Consult your doctor if your body reacts abnormally after sipping caffeinated drinks.

Experiment with different amounts until you know which caffeine is ideal for you. 

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Disclosure: No compensation or free products were received in exchange for writing this review.


Editorial Staff

The editorial staff at Crazy Coffee Crave is a team of coffee enthusiasts & Baristas who enjoy the one thing we all think about as soon as we get up in the morning. Trusted by thousands of readers worldwide.